Screening for Mutations in the GH-1 Gene by Dideoxy Fingerprinting (ddF)
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چکیده
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Single-strand conformation polymorphism (SSCP) (4) is the most widely used DNA screening method. SSCP detects single-base sequence changes by abnormal electrophoretic migration of one or both single strands on a nondenaturing gel. The method does not detect all sequence changes, and the sensitivity is a complex function of sequence context and size (1). Dideoxy fingerprinting (ddF) and bi-direc...
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15 صفحه اولScreening of two closed mutations in CFTR gene of Iranian infertile men with non-obstructive azoospermia
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ژورنال
عنوان ژورنال: Endocrine Journal
سال: 1999
ISSN: 0918-8959,1348-4540
DOI: 10.1507/endocrj.46.suppl_s71